Correlative microscopy

Spatial and temporal correlation

Open new perspectives with multi-modal acquisitions with our DHM® microscopes or cameras.
By combining Quantitative Phase Imaging (QPI) and fluorescence you obtain better insights into your monitored biological processes.

  • Quantitative Phase Imaging (QPI) offers label-free measurements of cell morphology and dry mass, two unique biomarkers for the cell’s physiological state
  • Fluorescence adds the specificity associated with the labeling of targeted cellular components or molecules

Spatial co-localization

Spatial co-localization thanks advantage of the specificity of fluorescence to identify distinct cellular structures.

Find more about how this spatial co-localization can be used to identify lipid droplets here.


Merged phase and fluorescence images of HeLa cells over-expressing alpha-Tubulin-GFP and H2B-mCherry
Spatial correlation between a specific fluorescent label for lipids (LipidTOX) and quantitative phase images obtained by DHM®.

Temporal co-localization

Temporal correlation between QPI and fluorescence provides valuable information on the dynamics of the monitored processes regarding the synchronization of the bio-processes.

Find more about how this temporal correlation can be used to investigate cardiomyocytes beating here.

Temporal correlation of cardiomyocytes beating recorded with DHM® (mechanical movement) and fluorescence (intracellular calcium release)