Electro-neutral ionic transport

Description

Neuronal ionic co-transporters NKCC1 and KCC2 are two neuronal co-transporters involved in Cl homeostasis and cell volume regulation. Dysfunction of these two co-transporters has consequences on neuronal excitability leading, in extreme cases, to pathogenesis of seizures and chronic neuropathic pain. The fact that NKCC1 and KCC2 co-transport have electro-neutral positive and negative charges makes the study by electrophysiology impossible.

Material and methods

  • Biological Model: Primary cultures of cortical neurons prepared from E17 OF1 mice embryos of either sex
  • DHM® solution: Non-invasive optical recording of neutral ionic co-transporters.

Results

DHM® is the first imaging technique offering the possibility to dynamically and directly monitor the activities of the two neuronal co-transporters NKCC1 and KCC2 in situ. Moreover, DHM® provides the possibility to pharmacologically characterize NKCC1 and KCC2. This is based on the fact that NKCC1 and KCC2 co-transport ions and water (up to 600 water molecule by charge), an underlying phenomenon which can be optical recorded by DHM®.

Figure 6 (adapted from Jourdain et al., J. Neurosc., 2011)

Publications

Jourdain et al., J. Neurosc., 2011

Biphasic response triggered by glutamate (30 µM, 30s, arrow head) in control condition (red line) and in the presence of furosemide (Furo., 100 µM, blueline). 2: RD response triggered by glutamate (30VM, 30s, arrow head) in control condition (red line) and in the presence of furosemide (blue line). 3: Concentration response curves of the inhibitory effect of furosemide on biphasic (full square and full line; IC50 = 580nM) and RD (empty square and dotted line; IC50 = 550nM)