Quantification of lipid droplets production plays a critical role in bone marrow transplantation as it can delay the onset of a proper immune response and prolong intensive care treatment.
The screen identified novel inhibitors and enhancers of adipogenesis and identified potential novel therapeutic candidates.
The specificity of the DHM signal was validated using a fluorescent dye specific to lipids. Then all experiments were done without fluorescence (cost saving and to prevent detachment of cells due to the washing step required by the fluorescence staining protocol)
This research was conducted by the group of Prof. Naveiras at EPFL and CHUV hospital, Switzerland
High-throughput, nonperturbing quantification of lipid droplets with digital holographic microscopy
V. Campos et al., J Lipid Res. 2018 Jul; 59(7): 1301–1310
My laboratory works on the regulation of the reversible transition between mammalian yellow (adipocyitic) and red bone marrow (hematopoietic), as this naturally occurring process can be enhanced to increase safety and efficacy of hematopoietic stem cell (HSC) transplantation.
We recently developed a novel assay with DHM to easily assess the production of intracellular lipid droplets. Lyncée Tec microscope provide a boost in the identification of potential modulators of adipogenesis that would be impossible to obtain with other techniques.