In-situ: cryo-DHM
DHM integrated in cryo-EM sample preparation workflow for ice-thickness mapping at cryo-temperature
Optimised sample preparation is essential for imaging biological macromolecules in their native state using single-particle cryo-electron microscopy (cryo-EM) or in situ cryo-electron tomography (cryo-ET). Gergely Papp’s Team at the European Molecular Biology Laboratory (EMBL) in Grenoble, France, and Lyncée Tec team have collaborated closely to integrate a DHM in their modular and automated EasyGrid platform, designed to streamline and standardize cryo-EM/ET sample preparation and quality control. Specifically, the DHM customised for this application provides fast, precise and efficient ice-thickness mapping at cryo-temperature to determine optimal grids for cryo-EM imaging, saving analysis time and costs.
Park systems Lyncée Tec division congratulates Gergely Papp and his team for the impressive publication of their work in a Nature Methods, that marks a significant milestone.
Description
- Courtesy of : European Molecular Biology Laboratory, EMBL, in Grenoble, France
- Material: grids for cryo-EM imaging
- Instrument: Customized DHM T-2100 mounted horizontally
- Magnification: 10x LWD
- Publication: Nat Methods (2026). https://doi.org/10.1038/s41592-026-03127-5
Partner Testimonial:
A long lasting and high technical and scientific collaboration
“We have collaborated with Lyncée Tec since 2017 to adapt holographic microscopy technology to cryogenic temperatures. Over the course of the project, several prototype iterations and customizations were developed to meet its demanding requirements, with both teams working closely together throughout the development process.“
Gergely Papp
European Molecular Biology Laboratory, EMBL, in Grenoble, France.
