Digital Holographic Microscopes (DHM®) have a unique optical configuration compared to classical interferometers: the reference path is not build-in the microscope objective but is integrated in the DHM® head. This enables DHM® to be used with a large range of objective types as conventional optical microscopes rather than to be limited by a limited choice of expensive interferometric Michelson or Mirau objectives. Therefore DHM® takes advantage of the multiple measurement possibilities offered with classical objectives.
The objective set selection chart on the left guides your choice.
Within a given set, objective selection criteria are identical to conventional optical microscopy. The objectives specification in term of magnification, field of view, lateral resolution (Numerical Aperture (NA)), maximum slope acceptance, working distance, are given for each sets of objectives in the related tabs at the top of this page.
Note that all the objectives within a same set have the same parafocal length, ensuring a constant focus without moving the sample when changing the objective. It is not possible to mount objectives from two different sets on a same DHM® system.
The objectives delivered are tested and selected for ensuring the best and optimal performances. They are factory adapted to the turret, calibrated and configured for an immediate use when a system is delivered.
Lyncée Tec experts will advise you on the best selection of objectives for your applications and samples.
Specifications for the standard set of objectives are divided into the following types:
The list below is not exhaustive. Do not hesitate to ask Lyncée for other application needs.
Magnification | Numerical aperture | Working distance [mm] | Glass thickness [mm] | Immersion | Field of View [um] (square) | Lateral resolution [um] | Depth of Field [um] | Maximum Slope [°] DHM®-R | Maximum Slope [°] DHM®-T |
---|---|---|---|---|---|---|---|---|---|
1.25 | 0.04 | 3.70 | - | - | 5280 | 8.50 | 555 | 1.1 | 4.5 |
2.5 | 0.07 | 11.2 | - | - | 2640 | 4.90 | 175 | 2 | 7.9 |
5 | 0.12 | 14.0 | - | - | 1320 | 2.85 | 58 | 3.4 | 13.3 |
10 | 0.30 | 11.0 | - | - | 660 | 1.15 | 9.75 | 8.7 | 28.7 |
20 | 0.40 | 1.15 | . | . | 330 | 0.85 | 5.0 | 11.7 | 34.3 |
40 | 0.75 | 0.37 | - | - | 165 | 0.45 | 1.43 | 24.3 | 41.8 |
50 | 0.75 | 0.50 | - | - | 132 | 0.43 | 1.23 | 26.6 | 41.8 |
100 | 0.90 | 1.0 | - | - | 66 | 0.38 | 0.91 | 32.1 | 41.8 |
Magnification | Numerical aperture | Working distance [mm] | Glass thickness [mm] | Immersion | Field of View [um] (square) | Lateral resolution [um] | Depth of Field [um] | Maximum Slope [°] DHM®-R | Maximum Slope [°] DHM®-T |
---|---|---|---|---|---|---|---|---|---|
20 | 0.40 | 10.80 | . | . | 330 | 0.85 | 5.0 | 11.7 | 34.3 |
50 | 0.50 | 8.20 | - | - | 132 | 0.70 | 3.0 | 15.0 | 38.2 |
100 | 0.75 | 4.70 | 66 | 0.45 | 1.3 | 24.3 | 41.8 |
Standard objectives with magnification smaller than 20x can be used for measurement through glass with optimal optical quality.
Magnification | Numerical aperture | Working distance [mm] | Glass thickness [mm] | Immersion | Field of View [um] (square) | Lateral resolution [um] | Depth of Field [um] | Maximum Slope [°] DHM®-R | Maximum Slope [°] DHM®-T |
---|---|---|---|---|---|---|---|---|---|
20 | 0.40 | 6.9 | 0-2.0 | . | 330 | 0.83 | 5.0 | 11.7 | 34.4 |
40 | 0.70 | 3.3--1.9 | 0-2.0 | - | 165 | 0.56 | 2.1 | 18.4 | 40.6 |
63 | 0.70 | 2.6 | 0.1-1.3 | - | 110 | 0.48 | 1.5 | 22.2 | 41.7 |
Water immersion objectives are available for Reflection DHM®. For transmission systems, immersion liquid holders have to be supplied by users.
Magnification | Numerical aperture | Working distance [mm] | Glass thickness [mm] | Immersion | Field of View [um] (square) | Lateral resolution [um] | Depth of Field [um] | Maximum Slope [°] DHM®-R | Maximum Slope [°] DHM®-T |
---|---|---|---|---|---|---|---|---|---|
10 | 0.30 | 3.6 | - | Water | 660 | 0.86 | 13.0 | 6.5 | 55.7 |
20 | 0.50 | 3.5 | . | Water | 330 | 0.51 | 4.5 | 11.0 | 61.8 |
40 | 0.80 | 3.3 | - | Water | 165 | 0.30 | 1.6 | 18.5 | 62.4 |
63 | 0.90 | 2.2 | - | Water | 110 | 0.30 | 1.3 | 21.3 | 62.4 |
10 | 0.40 | 0.36 | - | Oil, Water, Glycerine | 660 | 0.56 | 8.91 | 7.65 | 62.4 |
20 | 0.40 | 0.23 | - | Oil | 330 | 0.56 | 7.69 | 7.65 | 62.4 |
50 | 0.85 | 0.14 | - | Oil | 132 | 0.27 | 1.66 | 17.06 | 62.4 |
100 | 1.32 | 0.3 | - | Oil | 66 | 0.17 | 0.67 | 30.30 | 62.4 |
Specifications for Ultra Long Working Distance (ULWD) set of objectives are divided into the following types:
ULWD objectives are available without restriction for Reflection DHM®. They can be mounted on transmission DHM® as well, but without objective turret.
The list below is not exhaustive. Do nt hesitate to ask Lyncée for other application needs.
Magnification | Numerical aperture | Working distance [mm] | Glass thickness [mm] | Immersion | Field of View [um] (square) | Lateral resolution [um] | Depth of Field [um] | Maximum Slope [°] DHM®-R | Maximum Slope [°] DHM®-T |
---|---|---|---|---|---|---|---|---|---|
2 | 0.055 | 34 | - | - | 3300 | 6.21 | 284.32 | 1.58 | 17.80 |
5 | 0.14 | 34 | - | - | 1320 | 2.44 | 44.05 | 4.02 | 38.30 |
5 | 0.21 | 25.5 | - | - | 1320 | 1.63 | 21.62 | 6.06 | 48.30 |
7.5 | 0.21 | 35 | - | - | 880 | 1.63 | 19.58 | 6.06 | 48.30 |
10 | 0.28 | 34 | - | - | 660 | 1.22 | 11.01 | 8.13 | 54.40 |
10 | 0.42 | 15 | - | - | 660 | 0.81 | 5.41 | 12.42 | 60.40 |
20 | 0.28 | 30.5 | - | - | 330 | 1.22 | 9,86 | 8.13 | 54.40 |
20 | 0.42 | 20 | . | . | 330 | 0.81 | 4.64 | 12.42 | 60.40 |
50 | 0.42 | 20.5 | - | - | 132 | 0.81 | 4.18 | 12.42 | 60.40 |
50 | 0.55 | 13 | - | - | 132 | 0.62 | 2.49 | 16.68 | 62.20 |
50 | 0.75 | 5.2 | - | - | 132 | 0.46 | 1.39 | 24.30 | 62.40 |
100 | 0.70 | 6 | - | - | 66 | 0.49 | 1.49 | 22.21 | 62.40 |
100 | 0.9 | 1.3 | - | - | 66 | 0.38 | 0.91 | 32.08 | 62.4 |
Standard objectives with magnification smaller than 20x can be used for measurement through glass with optimal optimal quality.
Magnification | Numerical aperture | Working distance [mm] | Glass thickness [mm] | Immersion | Field of View [um] (square) | Lateral resolution [um] | Depth of Field [um] | Maximum Slope [°] DHM®-R | Maximum Slope [°] DHM®-T |
---|---|---|---|---|---|---|---|---|---|
20 | 0.28 | 29.42 | 3.5 | . | 330 | ||||
50 | 0.50 | 13.89 | 3.5 | - | 162 |
Magnification | Numerical aperture | Working distance [mm] | Glass thickness [mm] | Immersion | Field of View [um] (square) | Lateral resolution [um] | Depth of Field [um] | Maximum Slope [°] DHM®-R | Maximum Slope [°] DHM®-T |
---|---|---|---|---|---|---|---|---|---|
2.5 | 0.12 | 8.7 | - | . | 2640 | 2.84 | 68.91 | 3.45 | 34.30 |
5 | 0.25 | 12.5 | - | - | 1320 | 1.37 | 16.08 | 7.24 | 52.20 |
10 | 0.50 | 1.6 | - | - | 660 | 0.68 | 4.02 | 15 | 61.80 |
20 | 0.75 | 0.6 | - | - | 330 | 0.46 | 1.64 | 24.30 | 62.40 |
This list is not exhaustive. Do not hesitate to ask Lyncée for other application needs.
Objective type: Fluotar. It ensure optimal use of the fluorescence module with your DHM®.
Magnification | Numerical aperture | Working distance [mm] | Glass thickness [mm] | Immersion | Field of View [um] (square) | Lateral resolution [um] | Depth of Field [um] | Maximum Slope [°] DHM®-T |
---|---|---|---|---|---|---|---|---|
5 | 0.15 | 13.7 | With or without coverglass | - | 1320 | 2.85 | 58 | 13.3 |
10 | 0.32 | 11.1 | 0.17 | - | 660 | 1.15 | 9.75 | 28.7 |
20 | 0.55 | 1.20 | 0.17 | . | 330 | 0.67 | 3.31 | 38.2 |
40 | 0.80 | 0.40 | 0.17 | - | 165 | 0.44 | 1.40 | 41.8 |
63 | 1.30 | 0.16 | 0.17 | Oil | 110 | 0.18 | 0.8 | 89.9 |
100 | 1.32 | 0.18 | 0.17 | Oil | 66 | 0.17 | 0.7 | 89.9 |
This list is not exhaustive. Do not hesitate to ask Lyncée for other application needs.
As with any optical method, the reflected signal (DHM®-R) or the transmitted signal (DHM®-T) acquired is determined by the surface structure and material reflective or transmission property: steep slopes on the sample surface and diffusive materials will limit the quality of the reflected signal that can be acquired by the objective.
When no immersion medium is specified, the specimen is supposed to be surounded by air.
Using a transmission DHM®, the maximum slope depends on the specimen’s refractive index. Use of immersion liquid with calibrated refractive index enables to increase maximum slop measured and measure high aspect-ratio elements.
Lyncée Tec experts will advise you on the best configuration and objectives for your applications and samples.