DHM®     DIGITAL HOLOGRAPHIC MICROSCOPY
 
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Life science applications
Living cells analysis
Real-time cell monitoring
Separate morphology and refractive index measurements

3D representation of the thickness of a living neuron cell imaged over 30 min in transmission configuration with DHM T1000.

Separate morphology and refractive index measurements

The DHM technology and a patented decoupling procedure provide without contrast agent, invasiveness or phototoxicity the real-time visualization (up to 10'000 fps) of the morphology and the refractive index (related to protein concentration) of biological living cells. The quantitative measurements of these cells characteristics makes the DHM T1000 an ideal tool for dynamic analysis of cell health status, biological effects due to natural or artificial stimulations (drugs, electrical, thermal) such as cellular swelling or modification of intracellular protein concentration.

 

Application Note:

Biological specimen 3D morphology and refractive index separation

Scientific publication:

"Measurement of the integral refractive index and dynamic cell morphometry of living cells with digital holographic microscopy", B. Rappaz, P. Marquet, E. Cuche, Y. Emery, C. Depeursinge, and P. J. Magistretti, Opt. Express 13 (23), 9361-9373 (2005) -  1374 kB (.pdf)
Multimedia files 1 - 843 kB, 2 - 739 kB, 3 - 863 kB

Conference Proceedings:

"Erythrocytes volume and refractive index measurement with a Digital Holographic Microscope",B. Rappaz, A. Barbul, F. Charrière, J. Kühn, P. Marquet, R. Korenstein, C. Depeursinge and P. Magistretti, SPIE Proceeding Photonics West San Jose 2007