DHM®     DIGITAL HOLOGRAPHIC MICROSCOPY
 
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Life science applications
Living cells analysis
Real-time cell monitoring
Separate morphology and refractive index measurements

Real-time 3D phase image of a living trypanosoma brucei imaged in transmission configuration with DHM T1000.

Real-time monitoring

The DHM system is an ideal tool for real-time cell monitoring because it allows, from a single image acquisition, non-invasive high-resolution imaging of living cells in their natural environment without use of a contrast agent. The real-time imaging (up to 15 fps for a 512x512 pixels image) and the postponed reconstruction (up to 10'000 fps by using high-acquisition rate camera) make the DHM instrument a system well suited for the dynamic analysis of morphological modification of cells due to natural or artificial stimulations such as drugs, electrical or thermal stimuli.

For example, neuron cell swelling induced by hypotonic stress was studied by the Brain Mind Institute (EPFL, Switzerland).

 

Application Note:

Real-time 3D monitoring of living biological specimens

Scientific publication:

"Total aberrations compensation in digital holographic microscopy with a reference conjugated hologram",  T. Colomb, J. Kühn, F. Charrière, C. Depeursinge, P. Marquet, and N. Aspert, Opt. Express 14, 4300-4306 (2006) - 839 kB (.pdf)
Multimedia files 1 - 2.37 MB,
"Measurement of the integral refractive index and dynamic cell morphometry of living cells with digital holographic microscopy", B. Rappaz, P. Marquet, E. Cuche, Y. Emery, C. Depeursinge, and P. J. Magistretti, Opt. Express 13 (23), 9361-9373 (2005) -  1374 kB (.pdf)
Multimedia files 1 - 843 kB, 2 - 739 kB, 3 - 863 kB