DHM®     DIGITAL HOLOGRAPHIC MICROSCOPY
 
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Life science applications
Living cells analysis
Real-time cell monitoring
Separate morphology and refractive index measurements

3D phase image of a living neuron cell imaged in transmission configuration with DHM T1000.

Living cells analysis

The DHM T1000 brings unique possibilities for 3D real time and strictly non invasive observations. Unstressed cells can be investigated in their physiological medium without contrast agent and with low light intensity. The dynamics of any cell can be observed at a nanometric scale resolution. The fast image acquisition rate allows observation of physiological and pathophysiological processes related to cellular morphological changes. The robustness permits stable long time observations.

For example, a group of researchers from UNIL has been able to observe nanometric morphological changes of neuronal cells due to an increase of their electrical activity.

DHM T1000 is non-invasive and provides information not available with other microscopic techniques.

 

Application Note:

Living cells analysis: shape and structure measurements

Scientific publication:

"Living specimen tomography by digital holographic microscopy: morphometry of testate amoeba",  F. Charrière, N. Pavillon, T. Colomb, T. Heger, E. Mitchell, P. Marquet, B. Rappaz, and C. Depeursinge, Opt. Express 14, 7005-7013 (2006) - 760 kB (.pdf)
Multimedia files 1 - 1.26 MB, 2 - 1.65 MB,
"Cell refractive index tomography by digital holographic microscopy",  F. Charrière, F. Montfort, J. Kühn, T. Colomb, A. Marian, E. Cuche, P. Marquet, and C. Depeursinge, Opt. Lett. 31 (2), 178-180 (2006) - 1.3 MB(.pdf)
"Measurement of the integral refractive index and dynamic cell morphometry of living cells with digital holographic microscopy", B. Rappaz, P. Marquet, E. Cuche, Y. Emery, C. Depeursinge, and P. J. Magistretti, Opt. Express 13 (23), 9361-9373 (2005) -  1374 kb (.pdf)
Multimedia files 1 - 843 kB, 2 - 739 kB, 3 - 863 kB
"Digital holographic microscopy: a noninvasive contrast maging technique allowing quantitative visualization of living cells with subwavelength axial accuracy", P. Marquet, B. Rappaz, P. J. Magistretti, E. Cuche, Y. Emery, T. Colomb and C. Depeursinge, Opt. Lett. 30 (5), 468-470 (2005) - 588 kB (.pdf)